Development and evaluation of fluorescent recombinase polymerase amplification (RPA)-based method for rapid detection of Necator americanus
The fluorescent RPA assay presents a rapid and dependable method for detecting N. americanus in fecal samples. Its high sensitivity and specificity provide significant utility for field surveillance and early identification of N. americanus infections. This advancement could facilitate the rapid molecular diagnosis of N. americanus disease in hookworm-endemic regions.
Authors
- Lele Huo
- Zengnan Mo (ORCID: https://orcid.org/0000-0002-3047-3138)
- Wei Hu (ORCID: https://orcid.org/0000-0001-8763-2753)
- Juan Liang (ORCID: https://orcid.org/0000-0002-3774-9301)
- Han-Yin Yang (ORCID: https://orcid.org/0000-0003-0953-9574)
- Bin Zheng (ORCID: https://orcid.org/0000-0003-3476-5936)
- Shu-Ning Yan
- Yujuan Shen
- Bin Xu (ORCID: https://orcid.org/0009-0009-4639-4256)
- Yuchun Cai
- Shuo Yang (ORCID: https://orcid.org/0000-0002-3791-5541)
Institutions
- National Institute for Parasitic Diseases (CN)
- Shanghai Jiao Tong University (CN)
- Fudan University (CN)
- Inner Mongolia University (CN)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2025-04-08
- DOI
- https://doi.org/10.1371/journal.pntd.0013007
- Primary Topic
- Parasites and Host Interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Major Science and Technology Projects of China