Etiologic Diagnosis of Pulmonary Infection using Sputum Samples and Quantitative Loop-Mediated Isothermal Amplification (qLAMP) in Tianjian, China

This study endeavors to assess the efficacy of quantitative loop-mediated isothermal amplification (qLAMP) technology in the diagnosis of postoperative pulmonary pathogens and to evaluate its performance with that of conventional bacterial culture methods. The study encompassed 580 patients with a cardiac history, from whom sputum samples were collected postoperatively for the purpose of conducting qLAMP and bacterial culture tests. The findings reveal that qLAMP is capable of rapidly and accurately detecting pulmonary pathogens. Furthermore, we conducted an analysis of the clinical characteristics of the patients in light of the qLAMP results, uncovering significant correlations between age, smoking index, and postoperative tissue oxygen saturation levels with the incidence of pulmonary infections. The findings emphasize the potential of qLAMP in enhancing the accuracy of postoperative pulmonary infection diagnoses, providing a new rapid diagnostic tool for clinical use.