Spectrophotometric Determination of Biotin in Drug Samples using Ligand Substitution Reaction

A spectrophotometric approach that is straightforward, efficient, highly sensitive and precise has been devised to quantify biotin (BTN) in both its pure state and pharmaceutical samples. The methodology relies on the inhibitory approach of BTN on the Hg(II) promoted ligand substitution (LS) reaction involving pyrazine (PYZ) and hexacyanoferrate(II). The process entails replacing cyanide in [Fe(CN)6]4- with PYZ, triggering the development of a complex [Fe(CN)5PYZ]3-. The complex demonstrates a significant absorption level at a specific wavelength of 370 nm. The established limit of detection for BTN is 0.075μg mL−1. Recovery experiments were conducted to confirm the precision and accuracy of BTN quantification. The suggested approach has been effectively utilized for the examination of BTN in pristine samples and various medications, demonstrating remarkable levels of precision and accuracy. The outcomes showed good agreement when compared to the findings of the official analytical method. The excipients typically employed in medicines do not exhibit any interference with the suggested methodology.