Design and Validation of a Simplified Method to Determine Minimum Bactericidal Concentration in Nontuberculous Mycobacteria
Preincubation of Sensititre™ microtiter plates did not alter the MIC values of the antibiotics included except for rifampicin, suggesting a loss of activity. MBC determination can be easily performed by the Reincubation method presented. MBC values provide useful additional information regarding MIC values since the MBC/MIC ratio reveals whether antibiotics have bactericidal or bacteriostatic activity according to the species, which is pivotal for selecting the most adequate antibiotic combination to ensure efficient treatment management.
Authors
- Montserrat Garrigó (ORCID: https://orcid.org/0000-0003-4431-4733)
- Diego Martínez (ORCID: https://orcid.org/0000-0001-8651-582X)
- Mateu Espasa (ORCID: https://orcid.org/0000-0003-4822-1024)
- Mariana Fernández-Pittol (ORCID: https://orcid.org/0000-0002-1685-7127)
- Griselda Tudó (ORCID: https://orcid.org/0000-0002-9241-7051)
- Lorena San Nicolás
- Sofía Narváez (ORCID: https://orcid.org/0009-0007-9029-2367)
- Marc Rubio (ORCID: https://orcid.org/0000-0002-4811-0642)
- Sara Batista
- Julian González-Martín (ORCID: https://orcid.org/0000-0001-7248-6706)
- Elena Garcia Losilla
Institutions
- Instituto de Salud Carlos III (ES)
- Hospital de Sant Pau (ES)
- Departament de Salut (ES)
- Hospital Clínic de Barcelona (ES)
- Barcelona Institute for Global Health (ES)
- Fundació Clínic per a la Recerca Biomèdica (ES)
- Consorci Institut D'Investigacions Biomediques August Pi I Sunyer (ES)
- Fundació de Recerca Clínic Barcelona-Institut d’Investigacions Biomèdiques August Pi i Sunyer (ES)
- Universitat de Barcelona (ES)
Publication Details
- Journal
- Antibiotics
- Published
- 2025-04-04
- DOI
- https://doi.org/10.3390/antibiotics14040381
- Citations
- 7
- Primary Topic
- Mycobacterium research and diagnosis
- Type
- article
- Field-Weighted Citation Impact
- 9.48