Establishment of an I-ELISA method based on multi-epitope fusion protein for diagnosis of human brucellosis
The fusion protein constructed using bioinformatics techniques, as the diagnostic antigen, showed significantly reduced cross-reactivity and enhanced specificity, improving diagnostic accuracy. This not only saves time but also avoids the preparation of LPS antigens, making the diagnostic process safer and more convenient.
Authors
- Yixiao Chen (ORCID: https://orcid.org/0000-0001-5637-4632)
- Meixue Yao
- Qinglin Pei (ORCID: https://orcid.org/0000-0002-6584-4859)
- Liping Guo (ORCID: https://orcid.org/0000-0003-2065-387X)
- Shiqi Zhao (ORCID: https://orcid.org/0000-0002-7208-7075)
- Xinru Qi (ORCID: https://orcid.org/0000-0001-5697-9789)
- Dehui Yin (ORCID: https://orcid.org/0000-0002-7164-9320)
- Qi Wu (ORCID: https://orcid.org/0000-0003-3562-6129)
- Y. G. Xie (ORCID: https://orcid.org/0009-0006-3489-5879)
Institutions
- Xuzhou Medical College (CN)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2025-04-07
- DOI
- https://doi.org/10.1371/journal.pntd.0012995
- Citations
- 3
- Primary Topic
- Brucella: diagnosis, epidemiology, treatment
- Type
- article
- Field-Weighted Citation Impact
- 7.06
Funders
- Jiangsu Commission of Health
- Qinglan Project of Jiangsu Province of China
- Xuzhou Science and Technology Bureau