Label-free two-photon morphofunctional tissue imaging for precision diagnosis and treatment

Our studies utilize label-free, two-photon microscopy to achieve high-resolution, morphofunctional imaging for precision diagnosis and treatment. This technique captures metabolic and morphological data without exogenous labels. Specifically, we characterize mitochondrial organization changes in vitiligo lesions that are abrogated only in patients undergoing succesful treatment. For cervical pre-cancer, metabolic reprogramming indicators distinguish high-grade lesions with high sensitivity and specificity. In vivo and upcoming endoscopic applications demonstrate the technique's potential for non-invasive, real-time diagnostics, improving outcomes in dermatology and oncology.