Molecular detection of Listeria monocytogenes in refrigerated raw milk and geospatial distribution in Brazilian small farms
Listeriosis is a zoonosis whose etiological agent is the bacterium Listeria monocytogenes. Milk and dairy products are considered excellent substrates for the development of L. monocytogenes. Using conventional PCR, L. monocytogenes was investigated in 102 samples of raw milk from bulk tanks of small farms. Also, microbiological analysis was performed in positive samples by PCR. The primers such as PRS, LM1/LM2, inlA, inlC, and inlJ were used for spatial analysis. PRS indicated a positivity of 20.58%, and inlJ and inlC primers indicated 21.57%. The primers inlA and LM1/LM2 were negative. Our findings indicate it was possible to achieve effective molecular detection of L monocytogenes in the tank milk samples evaluated, in detriment to microbiological culture, in which there was no isolation.
Authors
- Amanda Bezerra Bertolini (ORCID: https://orcid.org/0000-0002-1575-6770)
- Laís Pereira da Silva (ORCID: https://orcid.org/0009-0003-8804-3813)
- Antônio Carlos Paes (ORCID: https://orcid.org/0000-0001-7774-927X)
- Virgínia Bodelão Richini‐Pereira (ORCID: https://orcid.org/0000-0001-8312-7397)
- Thassya Matias Ribeiro
- Simone Baldini Lucheis (ORCID: https://orcid.org/0000-0002-4842-5281)
- Isabella Neves Aires (ORCID: https://orcid.org/0000-0001-5285-3150)
- Rafael Fagnani (ORCID: https://orcid.org/0000-0002-7392-2087)
- Juliano Gonçalves Pereira (ORCID: https://orcid.org/0000-0002-8713-7506)
- Jackieline Sampaio Steinle (ORCID: https://orcid.org/0000-0002-7366-2449)
- Bruna Dos Santos Leite (ORCID: https://orcid.org/0009-0007-3138-8887)
- Vera Cláudia Magalhães Curci
- Marcos Vinícius marcosvrangel.ila gmail.com Rangel (ORCID: https://orcid.org/0000-0002-8191-2268)
- Thainá thainabertozzo gmail.com Valente Bertozzo
Institutions
- Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia (BR)
- Universidade Estadual Paulista (Unesp) (BR)
Publication Details
- Journal
- Food Science and Technology
- Published
- 2025-04-07
- DOI
- https://doi.org/10.5327/fst.00427
- Citations
- 1
- Primary Topic
- Listeria monocytogenes in Food Safety
- Type
- article
- Field-Weighted Citation Impact
- 1.23
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo