Efficient genetic transformation and genome editing via an <i>Agrobacterium</i>‐mediated in commercial oat (<i>Avena sativa</i> L.) cultivars
An optimized Agrobacterium-mediated transformation protocol for immature and mature oat embryos increased transformation efficiencies and the number of transformable cultivars and enabled highly efficient CRISPR/Cas9 and CRISPR/Cas12i genome editing to accelerate oat biotechnology breeding.
Authors
- Ke Wang (ORCID: https://orcid.org/0000-0001-6776-4935)
- Zhizhen Duan (ORCID: https://orcid.org/0000-0002-3498-754X)
- Zan Wang (ORCID: https://orcid.org/0000-0003-1114-9727)
- Weihong Huang
- Kun Shi (ORCID: https://orcid.org/0000-0001-8384-816X)
- Chenchen Zhu (ORCID: https://orcid.org/0009-0004-6119-5697)
- Shouzhen Teng (ORCID: https://orcid.org/0009-0000-0905-8012)
- Jinli Zhang (ORCID: https://orcid.org/0000-0002-3221-5167)
- Tiandou Hu (ORCID: https://orcid.org/0000-0003-0726-6621)
- M. Zhu (ORCID: https://orcid.org/0000-0003-4022-5899)
Institutions
- Chinese Academy of Agricultural Sciences (CN)
- Institute of Crop Sciences (CN)
- China Agricultural University (CN)
- Lanzhou University (CN)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2025-04-07
- DOI
- https://doi.org/10.1111/jipb.13915
- Citations
- 3
- Primary Topic
- Plant tissue culture and regeneration
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- China Agricultural University
- Shanghai Normal University