Development of a Fast and Quantitative Method to Evaluate Oocytes in Experimental Rabbits

The pathological evaluation of ovaries is a critical endpoint in reproductive toxicology assessments. Currently, there is no standardized method for the quantification of oocytes and follicles in preclinical drug safety evaluations. This study developed a simplified quantitative method based on stereological principles to efficiently assess the number of oocytes in experimental rabbits. The quantitative results obtained with this method are consistent with those from traditional counting methods. This innovative approach reduces the time of slide preparation and cell quantification, making it efficient for evaluating oocyte quality in large scale of toxicity studies.