Caging Gammarus roeseli to track pesticide contamination: How agricultural practices shape water quality in small waterbodies?

Contaminant monitoring in agroecosystems is increasingly revealing overlooked molecules, particularly within complex pesticide mixtures. This study assessed the effectiveness of chemical and ecotoxicological methods for evaluating contamination and biological responses in Gammarus roeseli exposed to pesticides and transformation products (TPs) in lentic small water bodies (LSWBs) near agricultural zones. We examined 7 LSWBs, finding variable contamination levels shaped by watershed composition differences. Analysis of 136 compounds identified key TPs, including chlorothalonil R471811, metazachlor ESA, and OXA, which collectively represented 86.2 % of the total quantified contaminants. These results underscore the persistence of both current and banned pesticides in the ponds studied. While G. roeseli showed favorable survival rates, significant reductions in locomotion and ventilation were observed at heavily contaminated sites, with biochemical analyses suggesting neurotoxic effects and activation of detoxification mechanisms in response to contaminants. Multivariate analyses revealed site-specific variations, highlighting the complex interactions between contamination levels and environmental conditions. Biomarker responses in gammarids served as sensitive indicators of residual toxicity in LSWBs, with frequent associations with historical contamination or current pesticide applications. This in situ caging approach across a contamination gradient demonstrates strong potential for biomonitoring and ecotoxicological assessments in agroecosystems. Extending exposure durations and including more heavily contaminated ponds could further enhance risk evaluation, thereby improving biomonitoring accuracy in headwater aquatic ecosystems. By integrating site-specific environmental conditions, contamination profiles, and biological responses, this study provides valuable insights into the influence of agricultural practices on LSWBs contamination and underscores the critical need to incorporate TPs into future risk assessment frameworks.