Formulation and Evaluation of Phytosome containing Trigonella foenum graecum Extract for in vitro Antioxidant and Anti-inflammatory Potential

The objective of current research work was to formulate Trigonella foenum graecum extract loaded phytosome and to evaluate its in vitro antioxidant and anti-inflammatory potential. The plant material was analysed for its quality and subjected for extraction. The extract was subjected for phytochemical investigations. Compatibility of drug and excipients was confirmed by FTIR. Phytosomes were prepared by thin film hydration method. The phytosomes were analysed for particle size and polydispersability index. The optimized formulation (TFG4) was subjected for in vitro antioxidant and anti-inflammatory assays. All physicochemical parameters were found to be within limit. The phytochemical investigation confirmed the presence of alkaloids, glycosides, steroids, terpenoids and phenols. The compatibility study confirms the compatibility of excipients and drug. Batch 4 (TFG4) exhibited smaller particle size of 244 nm and PDI of 0.5. With an IC50 value of 54.17 μg/ml, it is proven to have beneficial effects as antioxidant which was compared with standard ascorbic acid with an IC50 of 34.50μg/ml. In vitro anti-inflammatory activity showed an IC50 value of 56.68 μg/ml for sample and 28.63 μg/ml for standard. It can be concluded that phytosomes loaded with Trigonella foenum graecum exhibited promising antioxidant and anti-inflammatory potential and can be used as treatment model with better therapeutic efficiency.