Effect of Radiation on Oral Cancer patients by 1H NMR based Metabolomic

Oral microflora is well-orchestrated and acts as an in-order defense mechanism for any infection related to oral disease. It has an encouraging outcome in terms of early recovery biomarkers. The saliva of oral cancer patients' subjects and controls have been evaluated by 1 H NMR spectroscopy in search of possible early metabolic differences that could be obtained to see the eradication of disease that favors the symbiotic condition. The study employed 1 H NMR spectroscopy on 102 human saliva samples (including case and controls) and their spectral data were further subjected to multivariate and quantitative analysis. The 1H NMR study of oral cancer samples shows clear demarcation and profound metabolic differences when compared with the controls. Several metabolites such as lactate, ethanol, succinate and glutamate were found to be of higher significance in oral cancer patients in contrast to controls. Significant metabolites could be considered as early repair markers for oral cancer patients as they are being restored to achieve symbiosis. The study, therefore, concluded the early recovery process of the diseased subjects with the restoration of possible metabolomic profiles similar to the controls.