<i>In vitro</i> antioxidant, antidiabetic, and anticoagulant effects of <i>Eriobotrya japonica</i> Lindl. leaf extract

In this study, we evaluated the in vitro antioxidant, antidiabetic, and anticoagulant effects of a 40% (v/v) ethanolic extract of Eriobotrya japonica Lindl.leaves.The extraction yield was 29.30.8%,with total polyphenol and flavonoid contents measured at 360.111.0g gallic acid equivalents/mg and 256.928.9g quercetin equivalents/mg, respectively.The extract exhibited antioxidant effects, including radical scavenging activity, reducing power, and nitrite scavenging activity, all of which increased proportionally with concentration.The EC 50 values for 2,2-diphenyl-1-picrylhydrazyl radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical scavenging were 24.90.4 and 23.50.4g/mL, respectively.Additionally, the EC 50 values for reducing power and nitrite scavenging were 30.50.3 and 279.12.7 g/mL, respectively.The extract demonstrated significant antidiabetic activity, with an IC 50 value of 38.30.9 g/mL for the inhibition of -glucosidase.Furthermore, at a concentration of 2.9 mg/mL, the extract prolonged thrombin time by 3.1-fold, indicating notable anticoagulant activity.In conclusion, the Eriobotrya japonica Lindl.leaf extract was rich in polyphenols and flavonoids, exhibiting excellent physiological properties in terms of antioxidant, antidiabetic, and anticoagulant activities.