A Facile <i>In-Situ</i> Synthesis and Characterizations of Silver-Graphene Oxide (AgGO) Nanocomposite Using Chives as a Reducing and Stabilizing Agent

The Silver-graphene oxide (AgGO) nanocomposite is highly regarded for controlling the biological activity of silver nanoparticles (AgNPs). Combining AgNPs with Graphene Oxide (GO) offers better stability than single AgNPs. Traditional chemical synthesis methods use toxic reducing agents, posing environmental risks. This study aims to synthesize AgGO nanocomposite using a green synthesis method with chives (Allium tuberosum) extract as a reducing and stabilizing agent. The reducing properties of chives extract are attributed to its sulfur-containing compounds play a crucial role in the reduction of silver ions to silver nanoparticles. AgGO nanocomposite was synthesized with different chives extract concentrations (0.5 M, 1.1 M, and 2.0 M) and confirmed using UV-Visible spectrometer, X-Ray Diffraction (XRD), and High-resolution transmission electron microscopy (HRTEM). The 1.1 M concentration was the most efficient, forming AgNPs at 430 nm with an average particle size of 18 nm. The antibacterial activity against E. coli and S. aureus was assessed, revealing that AgGO-1.1 has significant antibacterial capabilities comparable to pure AgNPs.