A Rapid <scp>F0 CRISPR</scp> Screen in Zebrafish to Identify Regulator Genes of Neuronal Development in the Enteric Nervous System
Our multistep, yet straightforward CRISPR screening approach in zebrafish tests the genetic basis of ENS developmental and disease gene functions that will facilitate the high-throughput evaluation of candidate genes from transcriptomic, genome-wide association, or other ENS-omics studies. Such in vivo ENS F0 crispant screens will contribute to a better understanding of ENS neuronal development regulation in vertebrates and what goes awry in ENS disorders.
Authors
- Julia Ganz (ORCID: https://orcid.org/0000-0002-9471-1828)
- Chuhao Nie
- Nora R. W. Straquadine
- Christina G. Liu
- Sara A. Cook
- Ann E. Davidson
- Matthew C. Spaulding
Institutions
- Concordia University (US)
- New England College (US)
- University of New England (US)
- University of Michigan (US)
- Michigan State University (US)
- Colorado State University (US)
Publication Details
- Journal
- Neurogastroenterology & Motility
- Published
- 2025-04-06
- DOI
- https://doi.org/10.1111/nmo.70009
- Primary Topic
- Congenital gastrointestinal and neural anomalies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Simons Foundation
- Michigan State University
- American Gastroenterological Association
- National Institutes of Health
- College of Engineering, Michigan State University
- Allergan