Label-free quantification of antibody–oligonucleotide conjugates with varying stoichiometries using capillary zone electrophoresis
We report a label-free capillary zone electrophoresis platform enabling quantitative analysis of antibody-oligonucleotide conjugate (AOC) heterogeneity with single-stranded oligonucleotide (21 nt) resolution. This methodology resolves free antibodies, oligonucleotides, and AOC subpopulations while precisely evaluating conjugation efficiency and oligonucleotide-to-antibody ratio (OAR) heterogeneity across three synthetic strategies.
Authors
- Qinglin Xia (ORCID: https://orcid.org/0000-0002-9599-7864)
- Dongke Liu
- Xiaoxia Zhang (ORCID: https://orcid.org/0000-0003-3569-3081)
- Mo Zhou (ORCID: https://orcid.org/0009-0002-8926-3722)
- Jiang Li (ORCID: https://orcid.org/0000-0003-2372-6624)
- Linjie Guo (ORCID: https://orcid.org/0009-0005-1159-3280)
- Hongxu Chen (ORCID: https://orcid.org/0000-0003-2377-5733)
- Yanli Wen
- Lele Wang (ORCID: https://orcid.org/0009-0008-3006-313X)
Institutions
- Shanghai University (CN)
- Shanghai University of Engineering Science (CN)
- Chinese Academy of Sciences (CN)
- Shanghai Institute of Measurement and Testing Technology (CN)
- Shanghai Institute of Applied Physics (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- The Analyst
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5an00214a
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00