Bottom‐emitting striped microLED array light source for uniform optical sectioning structured illumination microscopy
Abstract We demonstrate a microLED light source for optical sectioning structured illumination microscopy (OS‐SIM). A bottom‐emitting design is employed to improve the uniformity and intensity of emission by removing the anode contact from the optical path. This design facilitates the addition of a potential equalizing and light‐reflecting layer to the top surface of the light source. The irradiance, uniformity of light emission, and contrast ratio of the light source are characterized. A system demonstration is performed with the light source integrated into a fluorescence microscope and used for OS‐SIM imaging.
Authors
- Juliet T. Gopinath (ORCID: https://orcid.org/0000-0003-2387-5371)
- Ioannis Kymissis (ORCID: https://orcid.org/0000-0001-7417-1759)
- Victor M. Bright (ORCID: https://orcid.org/0000-0001-5498-3071)
- Oliver Durnan (ORCID: https://orcid.org/0000-0002-3117-3487)
- Catherine A. Saladrigas (ORCID: https://orcid.org/0000-0001-5060-1996)
- Vikrant Kumar (ORCID: https://orcid.org/0000-0002-0745-5433)
- Ikponmwosa Stephen Ogunmwonyi
Institutions
- University of Colorado Boulder (US)
- Columbia University (US)
Publication Details
- Journal
- Journal of the Society for Information Display
- Published
- 2025-04-06
- DOI
- https://doi.org/10.1002/jsid.2069
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- National Institutes of Health