Larvicidal activity of <i>Magonia pubescens</i> A. St.-Hil. (Sapindaceae) seed and leaf extract on <i>Aedes aegypti</i>

Plant-derived compound emerges as alternatives to integrated control of <i>Aedes aegypti</i>, the main transmitter of Chikungunya, Dengue, Zika virus. The effects of <i>Magonia pubescens</i> A. St.-Hill extracts on <i>Ae. aegypti</i> larval development were assessed. Four <i>in vitro</i> assays analysed the insecticidal potential (% larval mortality) of aqueous and ethanolic (EE) extracts of leaves and seeds. The extracts were analysed using UHPLC-MS/MS. By comparing to data from three databases, 12 compounds from different classes were identified in the EE, including coumarins, phenolic acids, polyphenols, and flavonoids. The EE from the leaves was the most effective, with an LC₉₀ of 0.262 mg/mL. Morphological alterations were observed in larvae exposed to the extracts, showing epithelial walls with extravasation of intestinal contents. These findings highlight the effectiveness of <i>M. pubescens</i> extract as a bioinsecticidal agent against <i>Ae. aegypti</i> larvae.