Bioactive deproteinized bovine bone mineral based on self-assembled albumin nanoparticles promoted bone regeneration via activation of Wnt/β-catenin pathway

One of the major problems faced with pre-implant bone reconstruction therapy is that large bone defects do not heal over time. Artificial bone graft materials, such as deproteinized bovine bone mineral, are commonly used in clinics. However, the lack of osteoinductive capacity and risk of post-implantation infections remain key limitations. Bioactive materials with strong bone formation and a high degree of biocompatibility are still needed. In this study, we synthesised bovine serum albumin nanoparticles (BNP) loaded with Tideglusib (TD), TD and BNP were bound together by self-assembly, and mixed with deproteinized bovine bone mineral (DBBM) to form a bone substitute material (TD-BNP@DBBM) that had low cytotoxicity, promoted cell proliferation and migration, induced cell differentiation, and regulated osteogenesis. In vitro, experiments suggested that TD-BNP@DBBM could promote osteoblast differentiation of MC3T3-E1 cells. In vivo, experiments demonstrated that TD-BNP@DBBM significantly accelerated bone reconstruction and enhanced bone healing in a rat cranial defect model. Furthermore, this result suggested a link between the Wnt/β-catenin pathway and the osteogenic effect, providing a basis for subsequent investigations into the mechanism of bone regeneration induced by osteogenic biomaterials. TD-BNP@DBBM might be a promising new approach for treating bone defects.