Integrating NMR and multi-LC-MS-based untargeted metabolomics for comprehensive analysis of blood serum samples
Using a single clinical serum aliquot for simultaneous untargeted profiling via NMR and multi-LC-MS represents a highly efficient alternative to current methods. This approach reduces sample volume requirements and substantially expands the potential for broader metabolome coverage. Our study offers comprehensive insights into the impact of sample preparation on complex metabolic biofluid profiles, highlighting the compatibility and complementarity of LC-MS and NMR in metabolomics research.
Authors
- John Walsby-Tickle (ORCID: https://orcid.org/0000-0002-1287-9580)
- Elisabete Pires (ORCID: https://orcid.org/0000-0001-8646-062X)
- Tereza Kačerová (ORCID: https://orcid.org/0000-0002-9477-0182)
- Fay Probert (ORCID: https://orcid.org/0000-0002-8580-2023)
- James S. O. McCullagh (ORCID: https://orcid.org/0000-0003-4733-1205)
Institutions
- University of Oxford (GB)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2025-04-06
- DOI
- https://doi.org/10.1016/j.aca.2025.343979
- Citations
- 10
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 8.06
Funders
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council