IMPACT OF BOSINTHETICALY DERIVED β-CHITOSAN TITANIUM NANOPARTICLES ON THEENHANCMENT OF CAUDAL FIN REGENERATION ZEBRAFISH MOD

Background: Tissue regeneration is a crucial biological process with significant implications for developmental biology and regenerative medicine. Zebrafish (Danio rerio), known for their remarkable regenerative abilities, serve as an excellent model for studying tissue regeneration, particularly through the caudal fin regeneration model. Materials and Methods: β-Chitosan-TiO₂ nanoparticles were biosynthesized using plant extracts and microbial cultures. Characterization was performed using SEM, UV-Vis and FTIR. Zebrafish caudal fin regeneration was assessed post-amputation, with nanoparticle treatment in 16 μg/L and 32 μg/L. Regeneration was analyzed via microscopic observation and gene expression. Data were statistically analyzed using ANOVA, considering p < 0.05 significant. Results: β-Chitosan-TiO₂ nanoparticles exhibited uniform size, high stability, and strong biocompatibility. Zebrafish treated with β-Chitosan-TiO₂ nanoparticles showed significantly enhanced caudal fin regeneration compared to controls. Gene expression analysis supported upregulation of regenerative markers. Regenerated fin length measurements showed a dose-dependent improvement, with optimal effects at moderate nanoparticle concentrations. No significant toxicity or abnormalities were observed in treated zebrafish. Statistical analysis confirmed significant differences (p < 0.05) between control and treated groups, demonstrating β-Chitosan-TiO₂ NPs' potential in tissue regeneration. Conclusion: β-Chitosan-TiO₂ nanoparticles significantly enhanced zebrafish caudal fin regeneration by promoting cell proliferation and angiogenesis without toxicity. These findings highlight their potential as a biocompatible and eco-friendly nanomaterial for tissue regeneration applications.