PREPARATION AND CHARACTERIZATION OF HYDROXYAPATITE AND CISSUSQUADRANGULARIS COMPOSITE ENTRAPPED HYDROXYPROPYL METHYLCELLULOSESCAFFOLD FOR BONE REGENERATION-AN INVITRO STUDY

Background: The integration of biomaterials, such as hydroxyapatite (HAp) and natural extracts like Cissus quadrangularis (CQ), into scaffolds can enhance osteogenic properties and support bone healing. This study evaluates the potential of an HAp-CQ composite embedded in a Hydroxypropyl methylcellulose (HPMC) scaffold for bone regeneration. Material and methods: The HAp-CQ composite was synthesized using a sol-gel method. HPMC scaffolds were prepared through a solvent-casting and particulate-leaching technique, followed by the incorporation of the HApCQ composite. To prepare the hydroxypropyl methylcellulose scaffold with the impregnation of Cissus quadrangularis (CQ) and hydroxyapatite (HAp), 1 gram of CQ was dissolved in 10 mL of distilled water and heated at 80°C for 24 hours. Following scaffold formation, FTIR (Fourier Transform Infrared Spectroscopy), contact angle, tensile strength testing and anti-microbial activity was compared between the HAp-CQ and HPMC+HAp+CQ scaffold. Results:The synthesized HAp-CQ composite displayed improved mechanical properties and bioactivity, with FTIR confirming functional group interactions. The tensile strength of the test group scaffold was 1.51MPa and the contact angle of the test group scaffold showed an average angle of 53.07 degrees. Conclusion: The present study showed that the HPMC scaffold with the impregnation of CQ and HAp showed high tensile strength and increase in hydrophilicity. The mechanical property was increased and the antimicrobial activity showed an enhanced zone of inhibition