Old hematopoietic stem cells retain competence to reconstitute a youthful B cell system that is highly responsive to protein-based vaccination
Both DO-HSCs and DY-HSCs established in the young recipient BM to a similar extend, suggesting that the concomitant reduction in the de novo reconstitution of CD19<sup>+</sup> B cells in DO-HSC vs. DY-HSC transplanted animals is specifically related to old HSCs. DO-HSCs and DY-HSCs reconstitute very similar unchallenged B cell systems that efficiently elicit antigen-specific IgG antibodies by protein-based vaccination. Old HSCs thus retain competence to reconstitute a youthful and functional B cell system, at least in the young environment of transplanted RAG1<sup>-/-</sup> mice. This suggests that it is primarily age-related factors, and not HSCs per se, that influence the composition and functionality of the old B cell system.
Authors
- Hartmut Geiger (ORCID: https://orcid.org/0000-0002-5794-5430)
- Bettina Moehrle
- Reinhold Schirmbeck (ORCID: https://orcid.org/0000-0001-6853-8091)
- P. Kunath
- Alina Seidel (ORCID: https://orcid.org/0000-0001-7975-3790)
- Vadim Sakk (ORCID: https://orcid.org/0000-0001-5797-5851)
- Jan Münch (ORCID: https://orcid.org/0000-0001-7316-7141)
- Dominik Pflumm
Institutions
- Universität Ulm (DE)
- University Hospital Ulm (DE)
Publication Details
- Journal
- Immunity & Ageing
- Published
- 2025-04-05
- DOI
- https://doi.org/10.1186/s12979-025-00507-x
- Primary Topic
- T-cell and B-cell Immunology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Massachusetts General Hospital
- Deutsche Forschungsgemeinschaft
- Harvard Stem Cell Institute