Affinity maturation and optimization of CD44v6-targeting antibodies for molecular radiotherapy
The IgG1 LALA format demonstrated reduced binding to Fcγ receptors, potentially improving the safety profile. UU-A-155 emerged as the lead candidate for clinical translation, showing superior performance in both affinity and tumor retention. Our findings highlight the importance of comprehensive in vitro and in vivo assessments in antibody development, and provides valuable insights into optimizing antibody-based molecular radiotherapy.
Authors
- Leif Dahllund
- Fredrik Y. Frejd (ORCID: https://orcid.org/0000-0002-6421-4142)
- Helena Persson (ORCID: https://orcid.org/0000-0002-2965-0395)
- Marika Nestor (ORCID: https://orcid.org/0000-0003-4662-3142)
- Anja C. Mortensen (ORCID: https://orcid.org/0000-0002-6771-3289)
- Camilla Hofström
Institutions
- Uppsala University (SE)
- Science for Life Laboratory (SE)
- KTH Royal Institute of Technology (SE)
Publication Details
- Journal
- Nuclear Medicine and Biology
- Published
- 2025-04-05
- DOI
- https://doi.org/10.1016/j.nucmedbio.2025.109012
- Citations
- 2
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 2.72