Residue of allocryptopine and protopine in tissues and eggs of laying hens using ultra-high performance liquid chromatography–tandem mass spectrometry

The residues of allocryptopine (ALL) and protopine (PRO) in the eggs and tissues of laying hens from the veterinary drug Bopp Total Alkali Powder® were evaluated. UPLC-MS/MS technology was used to accurately determine these components. The study found that at a dose of 400 mg/kg, PRO in eggs reached a peak of 2.8 µg/kg on the seventh day of administration and was undetectable five days after stopping the medication. ALL was not detected throughout the study. At a dose of 2000 mg/kg, one day after stopping the medication, the residues of PRO and ALL reached 16.72 µg/kg and 8.61 µg/kg, respectively. Seven days after stopping the medication, PRO was undetectable, but there was still a residue of ALL. Tissue samples showed that two hours after stopping the medication, the concentrations of both components significantly increased in all tissues but rapidly declined within 24 h. Seven days after stopping the medication, no residues were detected in all tissues. The study suggests that the risk of residue is low when using Bopp Total Alkali Powder® at a dose of 400 mg/kg. However, using a dose of 2000 mg/kg may increase the risk of residue, so it is recommended to stop the medication for at least seven days to ensure food safety.