Single-cell spatial transcriptome atlas and whole-brain connectivity of the macaque claustrum
Claustrum orchestrates brain functions via its connections with numerous brain regions, but its molecular and cellular organization remains unresolved. Single-nucleus RNA sequencing of 227,750 macaque claustral cells identified 48 transcriptome-defined cell types, with most glutamatergic neurons similar to deep-layer insular neurons. Comparison of macaque, marmoset, and mouse transcriptomes revealed macaque-specific cell types. Retrograde tracer injections at 67 cortical and 7 subcortical regions defined four distinct distribution zones of retrogradely labeled claustral neurons. Joint analysis of whole-brain connectivity and single-cell spatial transcriptome showed that these four zones containing distinct compositions of glutamatergic (but not GABAergic) cell types preferentially connected to specific brain regions with a strong ipsilateral bias. Several macaque-specific glutamatergic cell types in ventral vs. dorsal claustral zones selectively co-projected to two functionally related areas-entorhinal cortex and hippocampus vs. motor cortex and putamen, respectively. These data provide the basis for elucidating the neuronal organization underlying diverse claustral functions.
Authors
- Changhong Zheng
- Ana Rita Ribeiro Gomes (ORCID: https://orcid.org/0000-0003-2010-0554)
- Fang Jiao (ORCID: https://orcid.org/0000-0001-5087-6228)
- Zihan Wu (ORCID: https://orcid.org/0000-0001-6342-9881)
- Wei Wu (ORCID: https://orcid.org/0000-0001-5643-8739)
- Xing Sun
- Zixian Yu
- Minyuan Zhang
- Cirong Liu (ORCID: https://orcid.org/0000-0002-7986-4615)
- Jianyun Ma
- Yanbing Lu
- Qian Yu (ORCID: https://orcid.org/0000-0003-4413-9744)
- Chengyu Li (ORCID: https://orcid.org/0000-0001-6829-0209)
- Xinxiang Song (ORCID: https://orcid.org/0000-0002-1674-1589)
- Yan-Gang Sun (ORCID: https://orcid.org/0000-0003-0768-7733)
- Zhiyue Lu (ORCID: https://orcid.org/0000-0002-0216-4346)
- Julien Vezoli (ORCID: https://orcid.org/0000-0001-8319-5429)
- Longqi Liu (ORCID: https://orcid.org/0000-0002-5828-5542)
- Pierre Misery
- Zhiyong Zhu (ORCID: https://orcid.org/0000-0002-6287-7743)
- Mu‐ming Poo
- Colette Dehay (ORCID: https://orcid.org/0000-0002-5196-1464)
- Yiqin Zuo (ORCID: https://orcid.org/0000-0002-7287-0350)
- Jiabing Liu (ORCID: https://orcid.org/0000-0002-2713-1049)
- Huanhuan Li (ORCID: https://orcid.org/0000-0003-1814-6182)
- Zhiming Shen
- Hanyu Shi
- Guangling Wang (ORCID: https://orcid.org/0009-0007-3283-2273)
- Linjie Lu (ORCID: https://orcid.org/0000-0003-1445-5201)
- Zhifeng Liang (ORCID: https://orcid.org/0000-0003-2758-1194)
- Anqi Zhao (ORCID: https://orcid.org/0000-0001-6006-9796)
- Ling Han (ORCID: https://orcid.org/0000-0002-3088-0374)
- Henry Kennedy (ORCID: https://orcid.org/0000-0002-2796-7663)
- Mengni Chen (ORCID: https://orcid.org/0000-0001-7018-8713)
- Cong Yu (ORCID: https://orcid.org/0009-0006-1702-8386)
- Biyu Ren (ORCID: https://orcid.org/0009-0009-7153-9322)
- Yanqing Zhong
- Yingjie An
- Ye Tian (ORCID: https://orcid.org/0000-0002-7359-3324)
- Qinwen Chai
- Yannong Dou (ORCID: https://orcid.org/0000-0002-9096-6284)
- Ying Xia (ORCID: https://orcid.org/0009-0007-6720-041X)
- Chun Xu (ORCID: https://orcid.org/0000-0001-5149-8257)
- Camille Lamy (ORCID: https://orcid.org/0009-0002-2106-1446)
- Ying Lei (ORCID: https://orcid.org/0000-0002-4349-3074)
- Zhaoke Luo (ORCID: https://orcid.org/0000-0001-8648-076X)
- S. D. Li
- Lingjun Ding (ORCID: https://orcid.org/0000-0002-9183-7062)
- Nini Yuan (ORCID: https://orcid.org/0000-0003-4623-3400)
- Chao Li (ORCID: https://orcid.org/0000-0002-8062-4539)
- Hui Zhang (ORCID: https://orcid.org/0000-0002-5300-5310)
- Jianfeng Liu (ORCID: https://orcid.org/0000-0003-3220-8941)
- He Wang (ORCID: https://orcid.org/0000-0002-1592-2564)
- Xin Li (ORCID: https://orcid.org/0000-0002-3575-9178)
- Qi Zhang (ORCID: https://orcid.org/0000-0002-5055-3637)
- Yiwen Liu (ORCID: https://orcid.org/0000-0003-2571-730X)
- Lina Wang (ORCID: https://orcid.org/0000-0003-1457-0015)
- Ruiqi Wang
- Yuan Liu (ORCID: https://orcid.org/0000-0003-4247-3562)
- Xinyan Zhang (ORCID: https://orcid.org/0000-0001-6269-3138)
- Kexin Wang (ORCID: https://orcid.org/0000-0003-1175-7829)
- Zhen Liu (ORCID: https://orcid.org/0000-0002-1886-6457)
- Yong Zhang (ORCID: https://orcid.org/0000-0002-5532-3654)
- Wei Zhang (ORCID: https://orcid.org/0009-0002-3507-4504)
- Xun Xu (ORCID: https://orcid.org/0000-0002-4246-4343)
- Yuxuan Liu (ORCID: https://orcid.org/0000-0003-4426-9290)
- Cui Wei
- Mingli Wang (ORCID: https://orcid.org/0000-0002-0343-0343)
- Jianhua Yao (ORCID: https://orcid.org/0000-0001-9157-9596)
- Shuzhen Zhang (ORCID: https://orcid.org/0009-0003-6688-4777)
- Shiwen Wang (ORCID: https://orcid.org/0000-0001-6294-4865)
- Yan Li (ORCID: https://orcid.org/0000-0003-2782-5443)
- Zhiyong Liu (ORCID: https://orcid.org/0000-0001-8257-4347)
- Jingjing Song (ORCID: https://orcid.org/0000-0002-5682-9550)
- Ruiyi Zhang (ORCID: https://orcid.org/0000-0002-4776-6762)
- Xiaofei Wang (ORCID: https://orcid.org/0000-0002-3248-6805)
- Yujie Hou
- Shuting Li (ORCID: https://orcid.org/0000-0003-3438-1588)
- Yun Zhao (ORCID: https://orcid.org/0000-0002-7807-6094)
- Shengjin Xu (ORCID: https://orcid.org/0000-0002-2388-3446)
- Yuanfang Xu (ORCID: https://orcid.org/0000-0002-9243-0192)
- Yaqian Wang (ORCID: https://orcid.org/0000-0002-7734-3787)
- Shengkang Li (ORCID: https://orcid.org/0000-0002-6864-5644)
- Huateng Cao
- Liangtang Chang
- Liqin Gu
- Xing Tan
- Haotian Yan
- Y. He (ORCID: https://orcid.org/0009-0009-8662-356X)
- Wanqiu Zhou
- Mingyuan Zheng
- Dafina Toplanaj
Institutions
- BGI Group (China) (CN)
- Université Claude Bernard Lyon 1 (FR)
- Inserm (FR)
- Shanxi Medical University (CN)
- Chinese Academy of Sciences (CN)
- Tencent (China) (CN)
- Shanghai Institute of Nutrition and Health (CN)
- Center for Excellence in Brain Science and Intelligence Technology (CN)
- Botswana Geoscience Institute (BW)
- Institut Cellule Souche et Cerveau (FR)
- Shanghai Center for Brain Science and Brain-Inspired Technology (CN)
- University of Chinese Academy of Sciences (CN)
Publication Details
- Journal
- Cell
- Published
- 2025-04-05
- DOI
- https://doi.org/10.1016/j.cell.2025.02.037
- Citations
- 24
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 19.34