SCREENING STUDY OF ANTIBACTERIAL ACTIVITY OF WATER-ETHANOLIC EXTRACTIONS OF POPULUS NIGRA L. AND POPULUS RUBRINERVIS HORT. ALB. BUDS

Today the search for new antimicrobial drugs is key to solving the global public health problem of combating antimicrobial resistance in the population. Medicinal plants are one of the most promising solutions to this problem. The aim of this study was to compare the antimicrobial activity of water-ethanolic extractions of some species of the genus Populus L. buds with the effect of propolis tincture as a comparison preparation. The minimum inhibitory concentration was determined by double serial dilutions on Mueller-Hinton nutrient broth (Bio-Rad, USA). The following clinical strains were used as test cultures: Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Candida albicans. It was revealed that the studied water-ethanolic extractions based of Populus nigra L. and Populus rubrinervis Hort. Alb. buds demonstrated antibacterial activity against clinical pathogens. Optimal extractants were selected to obtain dosage forms on the basis of the studied objects: with respect to Gram-positive bacteria S. aureus and B. cereus, stronger alcohols of 80 and 96% were proposed of P. rubrinervis Hort. Alb. buds and 70% of P. nigra L. buds; with respect to yeast-like fungus C. albicans, stronger 96% alcohol was required of P. rubrinervis Hort. Alb. buds and more hydrophilic 70% of P. nigra L. buds on the contrary. For both the Gram-negative bacteria P. aeruginosa and E. coli, the results of all study sites were comparable to the "negative" control. The antimicrobial activity of the comparison preparation - propolis tincture against the bacterium B. cereus was higher than against other clinical strains cereus was higher than against other clinical strains. This study indicates the promise of a further study P. rubrinervis Hort. Alb. buds. The buds of P. nigra L. and P. rubrinervis Hort. Alb. have been proposed as promising sources for obtaining medicinal plant preparations with antibacterial activity.