OPTIMIZATION OF THE METHOD OF ULTRASONIC EXTRACTION OF BIOLOGICALLY ACTIVE COMPOUNDS WITH A WATER-ALCOHOLIC MIXTURE FROM THE FLOWERS OF ARNICA MONTANA L., GROWING IN THE TERRITORY OF THE ARCTIC ZONE OF THE RUSSIAN FEDERATION UNDER INTRODUCTION CONDIT

Mountain Arnica (Arnica montana L.) is a longterm specially protected herbaceous species of the genus Arnica (Arnica L.) of the family of compoundflowers (Asteraceae Bercht. & J. Presl), growing mainly in forests and forest meadows of Western and Central Europe, in mountainous areas rising to the Alpine belt and successfully introduced into the Arctic zone of the Russian Federation. The inflorescences of the plant have hemostatic, anti-inflammatory, wound healing, analgesic, anti-sclerotic properties due to the high content of phenolic compounds, the complete extraction of which requires the development of conditions aimed at obtaining extracts containing the maximum number of target components with minimal destruction. In connection with the prospects of using the inflorescences of arnica plants in the field of healthcare, in this work, for the first time, the optimization of the conditions of the method of ultrasonic extraction with an aqueous ethanol mixture using single-factor analysis and the Box-Benken algorithm was carried out. The kinetics of the extraction process is approximated by the second-order reaction equation. The optimal duration of extraction, the concentration of the extractant, the power of ultrasonic action, the diameter of the fractions of vegetable raw materials, the hydromodule and the extraction temperature were determined. The content of polyphenols, flavonoids, and phenolic acids was determined, antioxidant activity and the degree of radical inhibition were evaluated. The results obtained can be used in the development of technologies for the production of phytopreparations for use in the pharmaceutical and cosmetology industries.