“A New era in bone regeneration: Blood Cell Progenerators for bone healing acceleration”

Aims: This study examined the impact of combining two platelet rich fibrin (PRF) regimens and CGFs (concentrated growth factors) with Osteon III (hydroxyapatite+ Beta-tricalcium-phosphate (HA/β-TCP composite)) on bone defect healing acceleration. Materials and methods: Five local breed male sheep have been used in the study. For experimentation, four 7-mm-diameter, 4-mm-deep bone defects were intentionally drilled in each sheep's tibia/radius. General anesthesia surgeries were spaced 2–4 weeks apart. The first distal defect was left untreated as a control, whilst the second defect was subjected to a treatment involving a combination of standard-platelet-rich fibrin (S-PRF) and Osteon III. The third defect was treated with Advanced-PRF (A-PRF) and Osteon III, while the fourth defect was treated with CGFs and Osteon III. Two weeks following the final surgery, each sheep was sacrificed. Computed tomography (CT) scanning was performed for measuring bone density using Hounsfield units at different time intervals. Results: The research demonstrated a notable increase in bone density as time passed in all groups (p < 0.001). Furthermore, the increase in bone density was considerably more pronounced in the CGF + Osteon III group compared to the A-PRF + Osteon III group, and it exceeded that in the S-PRF + Osteon III and control groups at all measured time points. This study indicates that the CGF + Osteon III group experienced a statistically significant enhancement in new bone formation when contrasted with the A-PRF, S-PRF, and control groups at all-time intervals. Conclusion: The results of this experimental research suggest that the inclusion of CGFs in Osteon III results in improved and expedited bone regeneration.