STANDARDIZATION OF RAW MATERIALS OBTAINED FROM AERIAL ORGANS OF NATURAL FIRE-BROAD (CHAMAENERION ANGUSTIFOLIUM L.)

Relevance. Raw materials obtained from the above-ground organs of narrow-leaved cypress (Ch. angustifolium) are used in folk medicine as an anti-inflammatory and antimicrobial agent. To date, there is no article on this species in the State Pharmacopoeia of the Russian Federation. For its development it is necessary to carry out standardization of raw materials. The aim is to carry out quantitative determination of BAV of Cypress narrow-leaved in above-ground organs in different phylogenetic phases for the purpose of standardization and introduction into ophicinal medicine. Methodology and scientific approaches. Harvesting of raw materials - herbs of Cypress was carried out according to pharmacopoeial methods. Numerical parameters of raw materials were determined. Tannins and vitamin C were determined by titrimetric methods. Flavonoids were analyzed using chromatography and differential spectrophotometry methods. The antioxidant activity of flavonoids was evaluated by deoxyribose method. Microscopic analysis of preparations of these raw materials was carried out to identify diagnostic features. Results of the study and conclusions. The content of vitamin C in the herb and flowers of freshly collected raw materials corresponds to the value of 22.48 and 30.73 mg%, respectively. The total content of flavonoids in the herb – 3.45%, tannins – 11.2%; anthocyanins in flowers – 0.05%. Rutin, quercetin were found in the herb of cypreus. Chrysanthemum was found in the flowers. The sum of flavonoids reduces the rate of hydroxyl radical generation by 56%. According to microscopy data, raphides were found in the herb as a diagnostic sign. Practical significance. The obtained data will allow to develop a pharmacopoeial article and use this raw material in ophicinal medicine.