VOLATILE COMPONENTS OF INULA HELENIUM AND I. GRANDIS, GROWING IN UZBEKISTAN

The composition of volatile components of essential oils and hexane extracts of the roots and essential oils of leaves of Inula grandis and Inula helenium, growing in Uzbekistan, was studied using the GC-MS method. In the essential oils of the roots of Inula grandis and Inula helenium, per 56 compounds were identified, which is 89.75 and 42.72% of the total amount of essential oils of the roots, respectively. In hexane extracts of Inula grandis and Inula helenium roots, 13 and 33 compounds were identified, accounting for 93.05 and 97.73%, respectively. The major components of the essential oil of Inula grandis roots are alantolactone (35.63%), igalan (15.77%), (+)-leden (7.91%), 4.15-dihydroisoalantolactone (7.01%), 6,12-epoxy-11β-eudesma-4,6-dien-3-one (5.40%), 11α,13-dihydroalantolactone (3.33%), а гексанового экстракта alantolactone (54.38%), igalan (17.16%), 6.12-epoxy-11β-eudesma-4,6-dien-3-one (6.85%), (+)-leden (6.79%), 11α,13-dihydroalantolactone (2.93%). It should be noted that the main components of the essential oil of Inula helenium roots with a content of 13.17 and 29.05%, and those of the hexane extract of Inula helenium roots were 13.70 and 29.53%, have not been identified. In the future, these components require isolation in their native form by chromatographic methods. The main components of roots Inula helenium essential oil are viridiflorol (3.39%), 1,5-cyclooctadiene, 3,4-dimethyl- (3.41%), 11α,13-dihydroelemasteiractinolide (2.85%), 11α,13-dihydroalantolactone (2.86%), alantolactone (10.92%), and the main components of roots Inula helenium hexane extract are (S*)-1-[(1S*,2S*)-2-ethenyl-1-methyl-2-(phenylthio)cyclopropyl] ethanol (14.77%), alantolactone (14.66%), phosphonic acid, [1-(1,1-dimethylethyl)-4,4-dimethyl-1,2-pentadienyl]- (5.31%), alloalantolactone (3.21%), 1-methyl-3,3-bis(trifluoromethyl) cyclohexene (3.08%). 45 compounds (70.7%) were identified in the essential oil of Inula grandis leaves, and 70 compounds (70.81%) were identified in the essential oil of Inula helenium leaves. Of the identified components of the essential oil of Inula grandis leaves are alantolactone (20.27%), isoalantolactone (7.81%), aromadendrene (3.63%), eudesma-4-en-3-one (3.11%), dihydroactinidioide (3.10%), and g-elemene (2.58%). Of the identified components of the essential oil of Inula helenium leaves are d-3-carene (7.70%), palmitinic acid (7.36%), benzene, 1,3-bis(1,1-dimethylethyl)-2-methoxy-5-methyl- (6.61%), β-ionone (3.90%), dihydroactinidiolide (2.83%), 3,7-benzofurandiol, 2,3-dihydro-2,2-dimethyl- (2.78%), diterpenoid phytene (2.62%). It has been confirmed that Inula grandis and Inula helenium are rich sources of sesquiterpenoids, especially sesquiterpene lactones of the germacrane, elemane and eudesmane types. It was revealed that essential oils of roots and leaves, as well as hexane extracts of Inula grandis and Inula helenium roots exhibit medium-level antibacterial and antifungal activity.