Sperm derived H2AK119ub1 is required for embryonic development in Xenopus laevis
Ubiquitylation of H2A (H2AK119ub1) by the polycomb repressive complexe-1 plays a key role in the initiation of facultative heterochromatin formation in somatic cells. Here we evaluate the contribution of sperm derived H2AK119ub1 to embryo development. In Xenopus laevis we found that H2AK119ub1 is present during spermiogenesis and into early embryonic development, highlighting its credential for a role in the transmission of epigenetic information from the sperm to the embryo. In vitro treatment of sperm with USP21, a H2AK119ub1 deubiquitylase, just prior to injection to egg, results in developmental defects associated with gene upregulation. Sperm H2AK119ub1 editing disrupts egg factor mediated paternal chromatin remodelling processes. It leads to post-replication accumulation of H2AK119ub1 on repeat element of the genome instead of CpG islands. This shift in post-replication H2AK119ub1 distribution triggered by sperm epigenome editing entails a loss of H2AK119ub1 from genes misregulated in embryos derived from USP21 treated sperm. We conclude that sperm derived H2AK119ub1 instructs egg factor mediated epigenetic remodelling of paternal chromatin and is required for embryonic development.
Authors
- José G. Abreu (ORCID: https://orcid.org/0000-0002-1363-1755)
- Jérôme Jullien (ORCID: https://orcid.org/0000-0002-7868-0021)
- Romain Gibeaux (ORCID: https://orcid.org/0000-0001-5081-1985)
- Jérémie Poschmann (ORCID: https://orcid.org/0000-0002-9613-5297)
- Vanessa Chenouard (ORCID: https://orcid.org/0000-0003-2385-8299)
- Valentin François-Campion (ORCID: https://orcid.org/0009-0009-3241-2281)
- Cynthia Fourgeux (ORCID: https://orcid.org/0000-0002-3330-7530)
- Kseniya Petrova (ORCID: https://orcid.org/0000-0002-6763-9744)
- Mami Oikawa
- Leonid Peshkin (ORCID: https://orcid.org/0000-0002-6420-848X)
- Maïssa Goumeidane
- François Berger (ORCID: https://orcid.org/0009-0002-8176-8830)
- Nolwenn Mouniée
Institutions
- Universidade Federal do Rio de Janeiro (BR)
- Centre National de la Recherche Scientifique (FR)
- Harvard University (US)
- Tokyo University of Pharmacy and Life Sciences (JP)
- Inserm (FR)
- Institut de génétique et de développement de Rennes (FR)
- Center for Systems Biology (US)
- Center for Research in Transplantation and Translational Immunology
- Université de Rennes (FR)
- Nantes Université (FR)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-04-05
- DOI
- https://doi.org/10.1038/s41467-025-58615-7
- Citations
- 1
- Primary Topic
- Epigenetics and DNA Methylation
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Agence Nationale de la Recherche
- National Institutes of Health
- Japan Society for the Promotion of Science