Self‐Assembled Fluorophore‐Based Probe for Efficient Detection of Endogenous Nitroreductase Activity in <i>Escherichia Coli</i> (Advanced Optical Materials 10/2025)

Stimuli-Responsive Supramolecular Cluster Based Fluorescent Probe A rational design strategy to construct stimuli-responsive supramolecular cluster based fluorescent probes, called “self-assembly induced lactone formation” (SAILac), has been developed. A new nitroreductase-reactive fluorescent probe, Rhodol-OBn(pNO2), is created based on the structure-activiy relationship studies of the fluorophore scaffold, resulting in a superior S/N ratio and higher reactivity toward nitroreductase than the original probe. This new probe successfully detects the activity of endogenous nitroreductase in Escherichia coli. More details can be found in article 2402530 by Eiji Nakata and co-workers.