Pyrrolidine Alkaloids and Polyketide Derivatives from the Marine-Derived Fungus <i>Trichoderma harzianum</i> ZN-4

Two unusual alkaloids with furanopyrrolidine skeleton, harzianopyrrolidones A-B (<b>1</b>-<b>2</b>), four new compounds, harzianopyridone B (<b>3</b>), harzianopyrone (<b>4</b>), (<i>R</i>)-1-(3,5-dihydroxy-4-methylphenyl)-1-hydroxyhept-5<i>E</i>-en-2-one (<b>5</b>), and harzianopyrrolidone C (<b>6</b>), together with two known compounds (<b>7</b>-<b>8</b>) were isolated from a marine-derived fungus <i>Trichoderma harzianum</i> ZN-4. Structures of the isolated compounds were elucidated based on their HRESIMS data, extensive NMR spectroscopic analyses, NMR calculation in combination with DP4+, ECD calculations, and a single-crystal X-ray diffraction experiment. New compounds <b>1</b>, <b>4,</b> and <b>5</b> exhibited potential cardiomyocyte protective activity at concentrations ranging from 5 to 20 μM. In addition, <b>7</b> showed moderate inhibitory activity against <i>Pestalotiopsis theae</i> with an MIC value of 25 μg/mL.