Scalable process development for <scp>rAAV</scp> transient transfection production using computational fluid dynamics modeling

Recombinant adeno-associated virus (rAAV) is a promising delivery vehicle for cell and gene therapies. Upstream development faces challenges like low productivity and inconsistent performance despite advancements. This study presents a scale-up design for robust rAAV production at 250 L scale using a transfection system. Initial process development in shake flasks optimized plasmid ratio to improve rAAV production. However, genome titer decreased by up to 50% in stirred-tank bioreactors, likely due to mechanical shear forces. Stirred-tank bioreactors were modeled with computational fluid dynamics (CFD) by M-STAR (250 mL, 5 L, 50 L) and with empirical correlations by Dynochem (250 L). Hydrodynamics were characterized to provide normalized shear stress across different geometries. The power per unit volume (P/V) of 71 W/m³ was optimal for the 250 mL bioreactor, focusing on cell growth, rAAV genome titer, capsid titer, and full capsid ratio. Based on CFD modeling, a P/V of 20 W/m³ was projected to perform best at 5 and 50 L scales during development, confirmed by comparable genome titer to low shear shake flask culture. A P/V of 15 W/m³ was subsequently projected for final production at the 250 L scale. The negative impact of shear stress could be further mitigated by adding extra Poloxamer-188 as a shear protectant. Additionally, pre-transfection viable cell density (VCD) was identified as a critical attribute. The final process included a 30% fixed-volume dilution of the cell culture along with controlled DNA complexation conditions to improve process robustness. Sequential production at the 250 L scale demonstrated consistent cell growth and rAAV production.