Isolation of Cyloeucalenol from Ethyl Acetate Extracts of Musa Acuminata Colla Bract

The investigation seeks to separate secondary metabolites to identify the bioactive compound(s) found in banana blossoms. The researchers divided the ethyl acetate extract into compounds using column chromatography. The fractions retrieved from the column were subjected to thin-layer chromatography to ensure their purity for component separation, yielding BF57 fractions as a white needle-like crystal substance. The proportion was determined using 1D Nuclear Magnetic Resonance (1D NMR) and 2D Nuclear Magnetic Resonance (2D NMR) spectroscopy. The extracted chemical structures were identified as cycloeucalenol after thoroughly interpreting NMR spectral data and comparison to the literature.