Optimisation of Heated Electrospray Ionisation Parameters to Minimise In‐Source Generated Impurities in the Analysis of Oligonucleotide Therapeutics
Optimisation of the mild source conditions in conjunction with high pH mobile phases was combined with high-resolution accurate mass spectrometry analysis and automated deconvolution workflows to develop a simplified and streamlined approach for characterising oligonucleotide therapeutics and their related impurities.
Authors
- Ulrik H. Mistarz (ORCID: https://orcid.org/0000-0003-2795-7550)
- Ken Cook (ORCID: https://orcid.org/0000-0001-5176-7736)
- Mark J. Dickman (ORCID: https://orcid.org/0000-0002-9236-0788)
- James L. Stephenson
- Mollie A. Glenister (ORCID: https://orcid.org/0009-0005-2994-1378)
Institutions
- Hemel Hempstead Hospital (GB)
- Thermo Fisher Scientific (Denmark) (DK)
- Thermo Fisher Scientific (United Kingdom) (GB)
- Thermo Fisher Scientific (United States) (US)
- University of Sheffield (GB)
Publication Details
- Journal
- Rapid Communications in Mass Spectrometry
- Published
- 2025-04-03
- DOI
- https://doi.org/10.1002/rcm.10033
- Citations
- 5
- Primary Topic
- DNA and Nucleic Acid Chemistry
- Type
- article
- Field-Weighted Citation Impact
- 4.03
Funders
- Directorate for Biological Sciences
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council