Ovarian tissue cryopreservation for fertility preservation: a two-decade single-center experience with 451 children and adolescents

Children and adolescents represent a small subset of OTC patients, with indications linked to common pediatric malignancies. For active storage ≥ 10 years, they store longer than adults, likely due to delayed reproduction or awaiting in vitro growth / in vitro maturation in hematological cases. Overall, adults store longer, but adolescent storage has risen over time possibly due to higher child mortality and previously limited OTC use in younger patients. Mean ovarian cortex surface data may guide pediatric tissue harvest recommendations, with unilateral oophorectomy advised. Fertility preservation counseling and cost coverage should be standard for pediatric patients undergoing gonadotoxic treatment. A tailored approach to OTC indications is essential, especially in high-mortality cancers like leukemia or sarcoma. Favorable pregnancy rates observed, even without OTT, suggest possible OTC overutilization, highlighting the need for individualized strategies and careful clinical decision-making to balance risks and preserve reproductive potential.