MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila

Nowadays, biological databases are playing an increasingly critical role in biological research. <i>Myceliophthora thermophila</i> is an excellent thermophilic fungal chassis for industrial enzyme production and plant biomass-based chemical synthesis. The lack of a dedicated public database has made access to and reanalysis of <i>M. thermophila</i> data difficult. To bridge this gap, we developed MTD (https://mtd.biodesign.ac.cn/), a cloud-based omics database and interactive platform for <i>M. thermophila</i>. MTD integrates comprehensive genome annotations, sequence-based predictions, transcriptome data, curated experimental descriptions, and bioinformatics analysis tools, offering a comprehensive, one-stop solution with a 'top-down' search strategy to streamline <i>M. thermophila</i> research. The platform supports data reproduction, rapid querying, and in-depth mining of existing transcriptome datasets. Based on analyses using data and tools in MTD, we identified shifts in metabolic allocation in a glucoamylase hyperproduction strain of <i>M. thermophila</i>, highlighting changes in fatty acid biosynthesis and amino acids biosynthesis pathways, which provide new insights into the underlying phenotypic alterations. As a pioneering resource, MTD marks a key advancement in <i>M. thermophila</i> research and sets the model for developing similar databases for other species.