Expression and characterization of cold-adapted xylanase Xyl-L in Pichia pastoris for xylooligosaccharide (XOS) preparation
The xylanase Xyl-L was successfully expressed in P. pastoris and proved to be able to degrade various flour substrates, producing XOS with DP ranging from 2 to 4, indicating its potential applications in the nutraceutical and food industries. Further studies must be performed to optimize its production in bioreactors.
Authors
- Sebastián Rodríguez (ORCID: https://orcid.org/0000-0002-7003-1764)
- Bárbara A. Andrews (ORCID: https://orcid.org/0000-0002-1001-2406)
- Brigitte Gasser (ORCID: https://orcid.org/0000-0003-2881-6370)
- Juan A. Asenjo (ORCID: https://orcid.org/0000-0002-3475-7664)
- Carolina González (ORCID: https://orcid.org/0000-0003-3566-4758)
- José Pablo Reyes-Godoy
Institutions
- Fraunhofer Chile Research Foundation (CL)
- Austrian Centre of Industrial Biotechnology (Austria) (AT)
- BOKU University (AT)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-04-05
- DOI
- https://doi.org/10.1186/s12934-025-02690-4
- Citations
- 6
- Primary Topic
- Biofuel production and bioconversion
- Type
- article
- Field-Weighted Citation Impact
- 4.04
Funders
- Amt der NÖ Landesregierung
- Bundesministerium für Klimaschutz, Umwelt, Energie, Mobilität, Innovation und Technologie
- Austrian Centre of Industrial Biotechnology
- Universität für Bodenkultur Wien
- Centre for Biotechnology and Bioengineering