Glucosinolate profiles in wild-growing <i>Lepidium draba</i> L. from Tunisia and Croatia

The glucosinolate (GSL) profiles in different plant parts of the wild-growing species <i>Lepidium draba</i> L. (syn. <i>Cardaria draba</i> Desv.) (Brassicaceae) from Tunisia and Croatia were determined using UHPLC-DAD-MS/MS. The predominant GSLs in both Tunisian and Croatian samples were glucoraphanin and glucosinalbin, with the leaves and flowers being the richest sources. The study also revealed previously unreported GSLs in <i>L. draba</i>, methionine-derived glucoiberin, glucoalyssin, glucohesperin, and glucolesquerellin, as well as tryptophan-derived glucobrassicin and 4-methoxyglucobrassicin. In addition to the variability of GSL content across different plant parts, this study has also shown the limitations of indirect GSL analysis by isothiocyanates using GC-MS, which can lead to incorrect identification of GSLs due to thermal degradation of the isothiocyanates produced.