QbD-Driven Development and Evaluation of EGCG-Loaded Transfersomal Gel for Non-Melanoma Skin Carcinoma

The most common type of cancer is non-melanoma skin cancer (NMSC), necessitating the development of non-invasive and novel method of treatment. The current research study is focused on the design, development, and evaluation of a transfersomal gel loaded with epigallocatechin-3-gallate (EGCG), a bioactive compound with significant anti-cancer properties. After establishing anticancer properties of EGCG using cell lines and in silico studies, transfersomes were formulated using Box-Behnken Design (BBD). Optimization was done with three factors and three levels The optimized transfersomal gel exhibited significant physicochemical characteristics. Smaller V.S., improved Z.P. and high EE facilitated enhanced skin penetration as proved by in vitro and ex vivo studies using porcine skin. These findings demonstrated the potential of transfersomal formulation in enhancing the transdermal delivery of EGCG, paving the way for effective approaches for managing