Na-Ag Ion-Exchanged Glass Substrates for Plasmon-Enhanced Fluorescence Imaging of Neutrophils
Here, we study the fluorescence response of neutrophils stained with the wheat germ agglutinin Alexa Fluor 594 dye when the cells are placed on plasmonic nanoparticle substrates. Specifically, we focused on gold and silver nanoparticles with particle sizes ranging from 12 to 250 nm. It was demonstrated that the intensity of fluorescence can be increased by more than 10 times when using substrates with silver nanoparticles formed by Na<sup>+</sup>-Ag<sup>+</sup> ion exchange in glass. The fluorescence enhancement depends significantly on both the size and surface density of the silver nanoparticles and the membrane staining procedure.
Authors
- Ekaterina Sherstyukova (ORCID: https://orcid.org/0000-0002-9962-6315)
- Snezhanna Kandrashina (ORCID: https://orcid.org/0000-0002-2185-3817)
- В. А. Сергунова (ORCID: https://orcid.org/0000-0002-8425-0845)
- Yevgeniy Sgibnev
- Alexander Baryshev (ORCID: https://orcid.org/0000-0001-9909-0349)
- Maxim Dokukin (ORCID: https://orcid.org/0000-0001-6856-1953)
- Vladimir Inozemtsev (ORCID: https://orcid.org/0000-0002-4693-5624)
- Artem V. Shelaev (ORCID: https://orcid.org/0000-0002-6864-0843)
- Н. В. Никоноров
- Mikhail Shvedov
Institutions
- ITMO University (RU)
- All Russia Research Institute of Automatics (RU)
- V.A. Negovsky Scientific Research Institute of General Reanimatology (RU)
Publication Details
- Journal
- Sensors
- Published
- 2025-04-03
- DOI
- https://doi.org/10.3390/s25072278
- Primary Topic
- Advanced Nanomaterials in Catalysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Russian Academy of Sciences