Synthesis and in vitro cytotoxic activity of novel Adamantane Carbohydrazide

A new 1-adamantyl derivative was developed, starting with adamantane-1-carbohydrazide as the bioactive core. The newly synthesized adamantane derivative N’-(4-hydroxy-3-methoxy-5-nitro benzylidene) adamantane-1-carbohydrazide (5-NVAC) was characterised employing diverse spectroscopic methods. The cell viability/cytotoxicity was identified through [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] MTT assay. The sample's cytotoxicity was assessed using the MTT assay within the HeLa cell line. In comparison to the control Doxorubicin, 5-NVAC demonstrated dose-dependent activity against the tested HeLa cell line after 48 hours of exposure. The lipophilic character of the adamantane moiety in 5-NVAC facilitates extensive hydrophobic interactions within the active site residues, alongside other types of interactions.