GC-MS analysis, antioxidant and antibacterial activities of fixed oil from <i>Cyphostemma digitatum</i> leaves

The current investigation was designed to explore the fixed oil derived from C. digitatum (leaves) and to evaluate its in vitro antioxidant and antibacterial properties for the first time. The fixed oil of C. digitatum was analyzed both qualitatively and quantitatively using a GC-MS instrument. The analysis revealed the presence of 28 saturated and unsaturated fatty acids. Among these, methyl ester of linolenic acid was detected in the highest concentration at 3.594%, followed by methyl ester of linoleic acid at 2.422%, palmitic acid at 2.213%, and oleic acid at 1.933%. The antioxidant activity of the fixed oil was evaluated against the DPPH radical using various concentrations of the oil in comparison with vitamin C. At a concentration of 100 µg/ml, the fixed oil exhibited a radical scavenging capacity of 67.574%. Additionally, the oil demonstrated significant antibacterial activity against S. epidermidis, S. aureus, S. typhimurium, and B. subtilis. The findings from this study suggest that the fixed oil of C. digitatum (leaves) represents a promising source of antibacterial and antioxidant agents