Development of Bio-Scaffold for the Treatment of 3º Burn Wounds

Wound healing, as a normal biological process in the human body, is achieved through four precisely and highly programmed phases: hemostasis, inflammation, proliferation, and remodeling. A better understanding of the influence of these factors on repair may lead to therapeutics that improve wound healing and resolve impaired wounds. In the present study we developed a gelatin-based bio scaffold for the treatment of wounds. Hemolytic assay was conducted to study the hemo-compatibility of bioscaffold towards the RBC cells. The results showed that the bioscaffolds were compatible towards the RBC cells with an IC 50 value of 124.2 µg/ml. Antiinflammation assay exhibited anti-inflammation activity maximum at the concentration of 500 µg/ml with an IC 50 value of 51.02 µg/ml. The bioscaffold showed a significant antibacterial and antifungal activity against human microbial pathogens: such as Propionibacterium acnes, Proteus vulgaris, E.Coli, Staphylococcus aureus, Candida albicans and Aspergillus niger. The efficiency of sample Gelatin+sample against Propionibacterium acnes and Staphylococcus aureus found to be high at the concentration of 500 µg/ml and the zone of inhibition was found to be 10 mm. The maximum zone of inhibition against fungal species were found to be 7 mm against Candida albicans at the concentration of 500 µg/ml. Taken together, these results showed that the gelatin-based bio scaffold could be a promising approach for the treatment of wounds.