Secondary Wound Healing Before and After Botox Injection

Aims: This study aimed to examine the effects of Botox therapy on the skin's wound regeneration that received the treatment before or after surgery.Materials and Methods: The 18 male albino rats weighing between (250-350) grams each got 1 IU of (Botox and saline) injected in the subcutaneous muscle in the center of 1.5 cm circles with 4.5 cm between them. Group A: injection seven days before surgery then animals had a full-thickness skin excision. Group B: injection immediately after wound incidence then all kept in separate cages. Each group was divided into three equal subgroups according to the healing period (3, 7, and 14 days) then histological tests were undertaken on skin biopsies for all the groups post-euthanasia.Results: There were significant differences between groups A and B On day three, group A exhibited mild inflammation, whereas groups B and both control groups had severe inflammation. On day 7, group A had scanty granulation tissue and re-epithelialization of more than half of the lesions. Group B had greater granulation tissue and less re-epithelialization. On day 14, both groups exhibited considerable granulation tissue growth, but group A had increased blood vessels and keratinocyte proliferation, which resulted in extremely good re-epithelialization and skin regeneration.Conclusions: We recommend that Botox injections be considered an effective alternative treatment option to accelerate wound healing. The most suitable method to administer Botox is before surgery.