Development of a novel Ga-containing hydroxyapatite/chlorhexidine biomaterial with antibacterial properties for future application in bone tissue engineering: an experimental and theoretical study
Abstract This study focuses on synthesizing gallium-containing hydroxyapatite (Ga-HA) with chlorhexidine (CLX) for potential use in bone and dental tissue restoration. The Ga-HA/CLX materials were prepared using a suspension-precipitation method and were surface-functionalized with varying CLX concentrations. X-ray diffraction analysis confirmed the hexagonal structure of Ga-HA with space group P63/m, while XPS revealed the presence of gallium and a Ca/P ratio ranging from 1.50 to 1.72. Infrared spectra exhibited characteristic bands for phosphate and CH2 groups, indicating CLX incorporation. The Ga-HA/CLX materials demonstrated 100% inhibitory efficiency against Staphylococcus aureus and Escherichia coli bacterial strains. MTT assay indicated enhanced cell viability in the presence of gallium, with the Ga-HA/CLX-0.20 material classified as non-toxic with 81.0 ± 3% cell proliferation. Density Functional Theory calculations supported favorable thermodynamics in the interaction between hydroxyapatite and chlorhexidine. Overall, Ga-HA/CLX materials exhibit promising properties for biomedical applications.
Authors
- Santiago Medina-Carrasco (ORCID: https://orcid.org/0000-0003-1353-9331)
- Anderson Gomes Vieira (ORCID: https://orcid.org/0000-0003-3102-1640)
- Josy Anteveli Osajima (ORCID: https://orcid.org/0000-0001-7089-3244)
- Ricardo Barbosa de Sousa (ORCID: https://orcid.org/0000-0002-5401-039X)
- Ewerton Gomes Vieira (ORCID: https://orcid.org/0000-0002-9537-2995)
- Maria G. Fonseca (ORCID: https://orcid.org/0000-0002-7818-4917)
- André Luiz Menezes de Oliveira (ORCID: https://orcid.org/0000-0002-7930-6234)
- Wiury Chaves de Abreu (ORCID: https://orcid.org/0000-0001-9889-7124)
- Edson C. Silva-Filho (ORCID: https://orcid.org/0000-0003-0988-2970)
- Marcos Pereira da Silva
- Régis Casimiro Leal (ORCID: https://orcid.org/0000-0003-3432-9288)
Institutions
- Universidade Federal da Paraíba (BR)
- Instituto Federal do Rio Grande do Norte (BR)
- Universidade Federal do Piauí (BR)
- Instituto Federal do Tocantins (BR)
- Instituto Federal do Maranhão (BR)
- Universidad de Sevilla (ES)
Publication Details
- Journal
- Cerâmica
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1590/ikwu3833
- Primary Topic
- Bone Tissue Engineering Materials
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa do Estado do Piauí
- Ministério da Ciência, Tecnologia, Inovações e Comunicações