Influence of the Anti-Osteoporosis Effect of Fish Collagen Supplement in Mice

Background: Osteoporosis (OA) is the second most serious epidemic worldwide in chronic progressive disease. Complex fish collagen powder contains astaxanthin extract, vitamin C, vitamin K1, vitamin D3, vitamin E, vitamin A, and whole milk powder to protect bone health. This novel combination for improving bone loss has not yet been studied or discussed. Objective: This trial investigated whether complex fish collagen (FC) powder containing astaxanthin extract, vitamin C, vitamin K1, vitamin D3, vitamin E, vitamin A, and whole milk powder had an improved effect on osteoporosis. Methods: The experiment has forty male Sprague-Dawley (SD) rats ages five weeks. The mice were divided into five groups (control, LCa+H2O, complex fish collagen doses 0.5-flod, 1-flod, and 2-flod) for twelve weeks. The complex fish collagen powder uses blood serum biochemical value, bone tissue, and biomechanics were analyzed. Results: Twelve weeks of low calcium feeding resulted in a significant reduction in the N-terminal telopeptides of type I collagen, the N-telopeptide (NTX) concentration, and the osteocalcin (OCN) concentration in the serum compared to the LCa+H2O group, the LCa+FC-L, LCa+FC-M, and LCa+FC-H group. According to the micro-computed tomography analysis, in comparison to the LCa+H2O group, the femoral bone mineral density, trabecular area percentage, and trabecular thickness of the LCa+FC-L, LCa+FC-M, and LCa+FC-H groups increased significantly, and the trabecular separation decreased significantly. Conclusion: By translating the experimental data into a daily supplement for adults, it is recommended that a daily intake of FC can help bone remodeling and bone strength, prevention effect on osteoporosis disease.