A novel boronic acid-based fluorescent sensor for the selective detection of <scp>l</scp>-lysine in food samples and cells
A novel probe DFC (2-(dicyanomethylene)-2,5-dihydro-5,5-dimethyl-4-((<i>E</i>)-2-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)furan-2-yl)vinyl)furan-3-carbonitrile) was successfully designed and synthesized for the detection of l-lysine (l-Lys). The sensing behavior was characterized using absorption and fluorescence emission spectra. Upon addition of l-Lys to DFC, a rapid response time of 5 seconds was observed, accompanied by a significant 4-fold enhancement in fluorescence intensity. Additionally, DFC exhibits an impressively low detection limit of 0.14 μMol L<sup>-1</sup>. Furthermore, the applicability of DFC was demonstrated through successful detection of l-Lys in water samples, food samples, and imaging of l-lys in live HeLa cells.
Authors
- Zhijun Wang (ORCID: https://orcid.org/0000-0003-2290-4986)
- Jingjing Liu (ORCID: https://orcid.org/0000-0001-5523-5534)
- Jing Su (ORCID: https://orcid.org/0000-0003-4866-6752)
- Xuefeng Zhao (ORCID: https://orcid.org/0000-0002-1047-1485)
- Qingming Wang (ORCID: https://orcid.org/0000-0001-9743-2069)
- Wei Gao (ORCID: https://orcid.org/0000-0003-3032-050X)
- Hae‐Won Yang
Institutions
- Changzhi University (CN)
- Yancheng Teachers University (CN)
Publication Details
- Journal
- RSC Advances
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5ra00621j
- Citations
- 1
- Primary Topic
- Molecular Sensors and Ion Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.76
Funders
- Shanxi Agricultural University
- Shanxi Academy of Agricultural Sciences
- Science and Technology Innovation Group of Shanxi Province